Edta induced clumping of lymphoid cells, both benign and malignant, in peripheral blood samples has been reported only rarely. A routine peripheral blood smear showed many intraleukocytic and extracellular cocci and diplococci figure. Peripheral blood smear examination macroscopic view. Schistocytes on the peripheral blood smear mayo clinic. Introduction peripheral blood smear is a very important tool in the hematology lab it provides rapid, reliable access to information about a variety of hematologic disorders examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and. Preparation of a peripheral blood film this protocol describes how to prepare a slide film of peripheral blood for microscopic examination. It remains a frontline diagnostic in unraveling mysteries behind cryptic symptoms and signs in primary and secondary haemopathies. Ppt peripheral blood smear powerpoint presentation free to. Complete blood cell count and peripheral blood film, its. Examination of peripheral blood smear powerpoint ppt presentation.
This synoptic reporting system for peripheral blood smears helps users to draft a complete, accurate, and concise report. In this video i quickly go over how to make a peripheral blood smear. Count 10 fields, take the total number of white cells and divide by 10. Examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and adults.
Detection of neuroblastoma cells in bone marrow and. Aims of this large descriptive repeated survey were to evaluate the diagnostic accuracy of different techniques in diagnostic samples obtained at several disease course time points and to correlate positive results with. Peripheral blood smear test about, preparation, test. Blood smears peripheral blood smear is a very important tool in the hematology lab it provides rapid, reliable access to information about a variety of hematologic disorders examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and adults the smear offers a window into the. Dlc by peripheral smear comparison with automated differential. Ppt peripheral blood smear morphology powerpoint presentation. Utmedical school at houston, pathology last revision on.
A 7monthold boy with a history of necrotizing enterocolitis and developmental delay. Staining process has begun, no stat slides can be added in the batch. Unexplained cytopenia anemia leukopenia thrombocytopenia pancytopenia unexplained cytosis leukocytosis blood smear. Peripheral blood smear demonstrating numerous echinocytes, among a few normal red blood cells from. View normal values, symptoms, peripheral blood smear test results, procedure to conduct. This way, each slide may be used to spread the film of the other, and duplicate slides will be of value if something happens to one of them in the. Detection of metastatic tumor cells in bone marrow bm and peripheral blood pb of children with neuroblastoma is crucial for prognosis and planning of therapy. Use the oil immersion lens to identify each cell type. Peripheral blood smear procedure and interpretation. Hematology powerpoint template powerpoint templates. Histoplasma capsulatum in peripheral blood smear bmj. Characteristic peripheral blood smear findings in disorders of. Choose a portion of the peripheral smear where there is only slight overlapping of the rbcs.
Abnormal monocytes on peripheral blood smears ppt xpowerpoint. The blood smear should occupy the central portion of the slide and should not touch the edges. It takes about 510 minutes to stain a batch of smears. The thickness of the spread when pulling the smear is determined by the 1 angle of the spreader slide the greater the angle, the thicker and shorter the smear, 2 size of the blood drop and 3 speed of spreading. The presence of schistocytes fragmented red blood cells on the peripheral blood smear suggests red blood cell injury from damaged endothelium and is a characteristic feature. Erythrocytes red blood cells, leukocytes white blood cells, and platelets can be easily identified using this slide. This picture illustrates the proper procedure for making blood smears. Volume of packed red blood cells per unit of blood, expressed as a percentage. In some ways it is becoming a lost art but it often provides rapid, reliable access to information about a variety of hematologic disorders.
Jul 15, 2019 a peripheral blood smear examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. I hope you can see the smear well enough in the video if not please leave a comment and i. Peripheral blood smear, preparation, examination, interpretation. A case of low grade b cell splenic lymphoma is presented with lymphocyte clumping in smears made from edta anticoagulated peripheral blood. View and download powerpoint presentations on peripheral blood smear evaluation ppt. The peripheral blood film pbf is a laboratory workup that involves cytology of peripheral blood cells smeared on a slide. May 01, 2015 peripheral blood smear examination macroscopic view. Find powerpoint presentations and slides using the power of, find free presentations research about peripheral blood smear evaluation ppt.
The organisms appeared purple in the wrightstained preparation, but. Slides are just automatically dipped in the stain in the buffer and a series of rinses. Estimates of cell count proportions of the different types of wbc morphology. At presentation, she was found to have severe pregnancy induced hypertension. The test provides information on the number and shape of these. As basic as it is, pbf is invaluable in the characterization of various. It is then treated with a special stain and studied under a microscope to examine the blood cells.
The cbc with differential presently includes a blood smear that is examined by the medical technologist and is available for the physician. Ppt examination of peripheral blood smear powerpoint. Such clustering presents the laboratory and pathologist with unique difficulties in the accurate diagnosis of these disorders. Peripheral blood smear dr ajit nambiar spherocytes causes hereditary spherocytosis immune hemolytic anemia severe burns invitro prolonged storage of. Ppt blood smear examination powerpoint presentation. Complete blood cell count and peripheral blood film can be truly diagnostic for a disease condition. Blood smear examination making blood smear manual differential counts these counts are done in the same area as wbc and platelet estimates with the red cells barely. Peripheral blood smear test purpose, procedure, results. Ppt blood smear examination powerpoint presentation, free. The examination of a peripheral blood smear is one of the most informative exercises a physician can perform. Trainees and practicing pathologists may benefit from this system, which is freely available on the internet. Ppt peripheral blood powerpoint presentation free to.
Blood hematopoiesislymphatics peripheral blood william f. Aqueous solutions of stains are stable only after 36. Kern, md director, laboratory hematology agglutination means the rbcs are sticking together in clumps not in. Hematology making a peripheral blood smear youtube. Synoptic reporting system for peripheral blood smear. The smear offers a window into the functional status of the bone marrow, the factory. Complete exhaustive presentation on peripheral blood smear exam. Meningococcemia diagnosed by peripheral blood smear jama. Peripheral blood smear dr ajit nambiar spherocytes causes hereditary spherocytosis immune hemolytic anemia severe burns invitro prolonged storage of blood. With the advent of hematological automated machines, complete and highly accurate hematological data and parameters can be obtained within a few minutes.
Echinocytes in the peripheral blood of patients infected with dengue. A free powerpoint ppt presentation displayed as a flash slide show on id. Interpreting peripheral blood smears harrisons principles. The three main blood cells that the test focuses on are. Peripheral blood smear introduction a properly prepared blood smear is essential to accurate assessment of cellular morphology the wedge smear is the most convenient. Jul 31, 2015 in this video i quickly go over how to make a peripheral blood smear. A blood smear is a blood test used to look for abnormalities in blood cells. It is found to reduce typing errors, enhance completeness, and improve reporting turnaround time. Find powerpoint presentations and slides using the power of, find free presentations research about abnormal monocytes on peripheral blood smears ppt. Smear peripheral blood seen under microscope stock illustration 20472289 find smear peripheral blood seen under microscope stock images in hd and millions of other royaltyfree stock photos, illustrations and vectors in the shutterstock collection.
A college student in previous good health presented with recent onset of fever, rigors, headache, and nuchal rigidity. A blood filmor peripheral blood smearis a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. I hope you can see the smear well enough in the video if not please leave a comment and i will try to make more visible slides. It is good practice to prepare two slides for each sample. The peripheral blood film pbf is a laboratory workup that involves cytology of. Notice the angle and direction that the spreader slide is pulled, first back towards. Malaria, dengue infection, ttp, dic, leukemia, apde. Atlas of hematology and analysis of peripheral blood smears. Splenic b cell lymphoma with lymphocyte clusters in. Peripheral blood film or peripheral blood smear or peripheral smear examination forms an important and basic lab investigation for diagnosing a number of hematological blood disorders. Nov 30, 2014 blood smears peripheral blood smear is a very important tool in the hematology lab it provides rapid, reliable access to information about a variety of hematologic disorders examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and adults the smear offers a window into the.
1399 1000 545 218 859 916 802 68 930 389 802 1342 608 35 578 340 522 1043 34 341 60 1067 913 932 1218 1455 217 935 755 363 75 181 1198 142 918 1318 947 780 868